*Laboratorio de Evolución, Facultad de Ciencias, Tristán Narvaja 1674, Montevideo 11200, Uruguay
Screening techniques for detecting allelic variation in DNA sequences
Article first published online: 14 APR 2008
Volume 2, Issue 2, pages 119–129, April 1993
How to Cite
LESSA, E. P. and APPLEBAUM, G. (1993), Screening techniques for detecting allelic variation in DNA sequences. Molecular Ecology, 2: 119–129. doi: 10.1111/j.1365-294X.1993.tb00006.x
This paper is the result of research carried out at the evolutionary genetics laboratory of the Museum of Vertebrate Zoology (UC Berkeley), where Lessa was a postdoctoral fellow and Applebaum an honors undergraduate student, both sponsored by Prof. James L. Patton. The efforts of the group are directed at the application of DNA techniques for the study of geographic variation, speciation and phylogeny of various mammalian groups.
- Issue published online: 14 APR 2008
- Article first published online: 14 APR 2008
- Received 26 May 1992; revision received 20 January 1993; accepted 23 January 1993
- allelic variation;
- heteroduplex analysis;
- screening techniques;
This article reviews four ‘DNA screening techniques’, namely heteroduplex analysis, single-strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.