Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst.

Authors


  • This paper reports the results obtained within a multigroup research effort on the population ecology of forest tree populations supported by the National Research Council of Italy. Gabriele Bucci has completed his graduate work for a PhD in Ecology writing a thesis on the use of RAPD markers in Picea abies populations ecology. Paolo Menozzi is Professor of Ecology, interested in population biology. His research spans a wide range of populations, from man to zooplankton to forest trees.

Prof. Paolo Menozzi

Abstract

The reliability of arbitrarily primed amplification products was tested. The segregation analysis of 266 amplification products obtained using 17 different 10-mer oligonucleotides in 34 megagametophytes from a single tree of Picea abies was carried out. Fifty-four out of the 165 variable bands fit the 1:1 segregation ratio expected for Mendelian traits. The segregation ratio of a subset of six RAPD markers in five other individuals from the same population confirmed their genetic nature. Our results strengthen the evidence previously reported that RAPDs markers can be considered Mendelian traits useful in the detection of genetic variability among both different individuals and populations.

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