This research arose from a collaborative project between the laboratories of Anna Glover, Jim Prosser and Ken Killham (Molecular & Cell Biology and Plant & Soil Science) to develop lux gene technology to enable single-cell detection of bacteria in environmental samples. Rosi Waterhouse was engaged on 6 years of postdoctoral research into the study of induced symbioses between bacteria and plants, and Deborah Silcock carried out 3 years' postdoctoral research into the development of a single-cell detection method using CCD technology. Hilary White completed a 1-year project investigating the usefulness of phage promoters to drive high expression of bacterial genes.
The cloning and characterization of phage promoters, directing high expression of luciferase in Pseudomonas syringae pv. phaseolicola, allowing single cell and microcolony detection
Article first published online: 14 APR 2008
Volume 2, Issue 5, pages 285–293, October 1993
How to Cite
WATERHOUSE, R. N., SILCOCK, D. J., WHITE, H. L., BUHARIWALLA, H. K. and GLOVER, L. A. (1993), The cloning and characterization of phage promoters, directing high expression of luciferase in Pseudomonas syringae pv. phaseolicola, allowing single cell and microcolony detection. Molecular Ecology, 2: 285–293. doi: 10.1111/j.1365-294X.1993.tb00021.x
- Issue published online: 14 APR 2008
- Article first published online: 14 APR 2008
- Received 8 January 1993; revision received 8 April 1993;accepted 27 April 1993
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