This paper is the result of a collaboration between B. R. Riddle and R. L. Honeycutt that began during Riddle's doctoral dissertation work. Data were generated in Riddle's molecular systematics laboratory at the University of Nevada, Las Vegas by P. L. Lee, a technician in the laboratory. This study represents one contribution to current attempts in this lab to understand the historical development of biotic associations in western North America, which includes ongoing studies on ecologically associated mammals, lizards, and plants.
Mitochondrial DNA phylogeography in northern grasshopper mice (Onychomys leucogaster) — the influence of Quaternary climatic oscillations on population dispersion and divergence
Article first published online: 14 APR 2008
Volume 2, Issue 3, pages 183–193, June 1993
How to Cite
RIDDLE, B. R., HONEYCUTT, R. L. and LEE, P. L. (1993), Mitochondrial DNA phylogeography in northern grasshopper mice (Onychomys leucogaster) — the influence of Quaternary climatic oscillations on population dispersion and divergence. Molecular Ecology, 2: 183–193. doi: 10.1111/j.1365-294X.1993.tb00107.x
- Issue published online: 14 APR 2008
- Article first published online: 14 APR 2008
- Received 2 September 1992; revised 2 March 1993; Accepted 18 March 1993
- mitochondrial DNA;
- northern grasshopper mice;
The details of mitochondrial DNA (mtDNA) phylogenetic structure of the northern grasshopper mouse Onychomys leucogaster were examined using populations from a postulated area of endemism that includes three arid regions (Colorado Plateaus, Interior Plains, and Wyoming Basins) in western North America. Fifteen tetra- and heptanucleotide restriction enzymes were used to assay restriction-site variation in a 2150-bp PCR-amplified fragment of mtDNA representing the ND2 and part of the COI gene regions. A total of 18 mtDNA haplotypes were detected. Although overall genetic divergence among these haplotypes was low (average = 1.1%), phylogeographic structuring was apparent. Notably, a clear phylogenetic split separated one group of haplotypes restricted to the Wyoming Basins from all others. This phylogenetic split was further corroborated by examination of nucleotide sequence variation from a 270-bp stretch of the mtDNA cytochrome b gene. Overall geographic and phylogenetic patterns suggest a complex history of geographic structuring and subsequent mixing of populations of grasshopper mice throughout the late Pleistocene. These patterns of variation are evaluated relative to alternative hypotheses about biotic responses to Quaternary climatic oscillations in western North American arid regions.