This paper is part of an extensive effort to evaluate the importance of bacteriophage-bacteria interaction in natural environments. Our laboratories are interested in assessing the potential of environmentally occurring bacteriophages to regulate both bacterial population densities and the makeup and diversity of natural microbial gene pools. S. Ripp is currently a postgraduate student in the laboratory of R.V. Miller.
Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1
Article first published online: 9 OCT 2008
Volume 3, Issue 2, pages 121–126, April 1994
How to Cite
RIPP, S., OGUNSEITAN, O. A. and MILLER, R. V. (1994), Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1. Molecular Ecology, 3: 121–126. doi: 10.1111/j.1365-294X.1994.tb00112.x
- Issue published online: 9 OCT 2008
- Article first published online: 9 OCT 2008
- Received 7 June 1993; accepted 1 September 1993
- aquatic environments;
- bacteriophage UT1;
- generalized transduction;
- horizontal gene transfer;
- Pseudomonas aeruginosa
A pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer of both chromosomal andplasmid DNA between strains of Pseudomonas aeruginosa. Several chromosomal alleles from three different P. aeruginosa strains were found to transduce at frequencies from 10-8 to 10-10 transductants per PFU at multiplicities of infection (MOD between 0.1 and 1. Transduction frequencies of certain alleles increased up to 1000-fold as MOIs were decreased to 0.01. UT1 is also capable of transducing plasmid DNA to indigenous populations of microorganisms in natural lake-water environments. Data obtained in this study suggest that environmentally endemic bacteriophages such as UT1 are formidable transducers of naturally occurring microbial communities. It should be possible to develop model systems to test transduction in freshwater environments using components derived exclusively from these environments.