A phylogenetic analysis of micro-organisms isolated from subsurface environments


  • K. P. Stim

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    1. Department of Biochemistry and Cell Biology — MS 140, 6100 S. Main Street, Rice University, Houston, TX 77005-1892, USA
      Tel. (713)527 8101 × 3304. Fax (713) 285 5154.
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  • This work is part of Kathleen P. Stim's PhD thesison the identification of unknown micro-organisms isolated from Southeast Coastal Plain subsurface sediments. It was under taken in David L. Balkwill's laboratory in the Department of Biological Sciencesat Florida State University.

Tel. (713)527 8101 × 3304. Fax (713) 285 5154.


Three methods were used to provide information on the identity and phylogenetic relatedness of 19 aerobic, chemoheterotrophic bacteria isolated from topsoil and deep subsurface sediments at a site in South Carolina. These methods were (i) analysis of selected physiological traits, (ii) restriction endonuclease analysis (REA) of genomic DNA, and (iii) analysis of 16S ribosomal RNA sequences. When the 16S rRNA sequences were compared with those for 12 standard strains, two topsoil isolates and six subsurface strains formed a tight group with the high-G+C Gram-positive bacteria and appeared to be most closely related to Arthrobacter globiformis— a coryneform-actinomycete bacterium with unusually effective survival capabilities. The rest of the subsurface isolates were scattered among the standard strains from the Proteobacteria— including the pseudomonads and Agrobacterium tumefaciens— or the low-G+C Gram-positive bacteria.