*Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK
Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies
Article first published online: 28 JUN 2008
Volume 4, Issue 2, pages 249–252, April 1995
How to Cite
PEMBERTON, J. M., SLATE, J., BANCROFT, D. R. and BARRETT, J. A. (1995), Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Molecular Ecology, 4: 249–252. doi: 10.1111/j.1365-294X.1995.tb00214.x
The authors' laboratory, which has recently moved to Edinburgh University, is mwmhing and implementing molecular methods for rapid, largescale screening of variation in natural populations. The work described here is part of a long-term, multi displinary to investigate the factors affecting reproductive success and the distribution of genetic variation in individually monitored red deer on the island of Rum; a study in collaboration with Dr T. H. Clutton-Brock and coworkers in the Department of Zoology, Cambridge and Dr S. D. Albon and coworkers at the Institute of Zoology, London.
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 5 May 1994 revised 17 July 1994 accepted 11 August 1994
- Cervus elaphus;
- nonamplifying alleles;
- red deer
While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAP65, BOVIRBP and CelJP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-offspring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.