*CSIRO Division of Entomology, PO Box 1700, Canberra, ACT 2601, Australia
Geographic and microgeographic genetic differentiation in two aphid species over southern England using the multilocus (GATA)4 probe
Article first published online: 28 JUN 2008
Volume 4, Issue 3, pages 375–384, June 1995
How to Cite
de BARRO, P. J., SHERRATT, T. N., CARVALHO, G.R., NICOL, D., IYENGAR, A. and MACLEAN, N. (1995), Geographic and microgeographic genetic differentiation in two aphid species over southern England using the multilocus (GATA)4 probe. Molecular Ecology, 4: 375–384. doi: 10.1111/j.1365-294X.1995.tb00230.x
Gary Carvalho, Norman Maclean and Steve Wratten developed the first aphid finger prints using the Jeffieys 33.15 human minisatellite core sequence. Paul De Barn has research interests in both aphid ecology and the application of molecular techniques to ecology. Until recently he was a Visiting Research Fellow in Norman Madean's Animal Genetics Research group, but he has now rewed to workonaphid geneticsin Australia. TomSherratt is an ecological modeller with a particular interest in the foraging behaviour of insects. DaveNicoland Arati lyengarare postdoctoral researchers within Norman Maclean's laboratory, working on aphid ecology and transgenic fish, respectively.
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 4 July 1994 revised 9 January 1995 accepted 6 February 1995
- Sitobion avenae;
- Metopolophium dirhodum;
- DNA fingerprinting;
- host preference;
- genetic variation
Samples of the grain aphid Sitobion avenue (F.) and the rose-grain aphid Metopolophium dirhodum (Walker) were collected in late March from wheat fields and adjacent road-side grasses at a number of locations in southern England. Unparasitized aphids were DNA fingerprinted using the multilocus (GATA)4 probe. Over all locations, the fingerprints of individual S. avenue caught in wheat had lower overall average distances of band migration (ADBM) and shared a higher proportion of bands, than fingerprints of individuals caught in adjacent road-side grasses. The ADBM of fingerprints of S. avenue collected on road-side grasses altered significantly with geographical location, while the ADBM of fingerprints of S. avenue caught on wheat did not. A comparison of the fingerprints of individual M. dirhodum caught in wheat and neighbouring road-side grasses did not reveal any genetic differentiation. Fingerprints of M. dirhodum that were caught in the same host type did however, show significant variation in ADBM between different locations. With both S. avenue and M. dirhodum, spatial autocorrelation revealed that locations that were close together were no more likely to have individuals with similar ADBM than locations mat were far apart Our results suggest that (i) particular clones of S. avenue prefer to colonize wheat; and/or that (ii) particular clones of S. avenae perform better on wheat man other clones. It is unclear why M. dirhodum did not show any genetic structuring according to host type, but this species appears to engage in sexual reproduction much more frequently than S. avenae in southern England. M. dirhodum is likely to have displayed genetic heterogeneity between locations either because of founder effects, or because of genetic drift.