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Keywords:

  • capelin;
  • cytochrome-b;
  • DNA sequencing;
  • mitochondrial DNA;
  • polymerase chain reaction;
  • restriction analysis

Abstract

Divergence estimates were calculated using restriction analysis and cytochrome-b sequences for capelin Mallotus villosus mitochondrial genomes sampled from Newfoundland and Norwegian populations. On average, pairwise divergence values based on cytochrome-b sequences were 2.7 times greater than those based on restriction data. Since the rate of evolution in the cytochrome-b gene is low, it appears that restriction analysis underestimated divergence values, possibly due to limited sensitivity of this method when variable nucleotide positions are not randomly distributed. Mitochondrial genotypes were not shared among the two populations.