Estimation of genomic variation between British populations of the potato cyst nematode Globodera pallida using RAPD-PCR

Authors


  • Dr Paul Burrows is a molecular biologist working on plant parasitic nematodes and their interactions with host plants. The molecular basis of nematode virulence and host resistance are of particular interest. Paul Halford and Dr Ken Evans are both concerned with the improvement of integrated control of Globodera pallida in the UK.

Tel.: +(0)1582–763133; Fax: +(0)1582–760981. E-mail: RFC-822: Paul.Burrows@bbsrc.ac.uk

Abstract

Random amplified polymorphic DNA (RAPD) has been used to investigate the interrelationships of 20 populations of Globodera pallida collected originally from field soils around the UK. RAPD analysis revealed a high level of relative genomic diversity within British G. pallida but there was no general correlation of genomic similarity with geographic distribution. Two populations of pathotype Pa1 were clearly divergent from the bulk of G. pallida and might represent a distinct introduction. Two other populations, from Scotland and Wales, were also dissimilar from each other and from the rest of the G. pallida populations.

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