Present address: Biodiversity and Ecological Processes Group, Cardiff School of Biosciences, Cardiff CF10 3TL, UK.
Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method
Article first published online: 20 MAY 2004
Volume 13, Issue 7, pages 2089–2094, July 2004
How to Cite
NSUBUGA, A. M., ROBBINS, M. M., ROEDER, A. D., MORIN, P. A., BOESCH, C. and VIGILANT, L. (2004), Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method. Molecular Ecology, 13: 2089–2094. doi: 10.1111/j.1365-294X.2004.02207.x
- Issue published online: 20 MAY 2004
- Article first published online: 20 MAY 2004
- Received 6 November 2003; revision received 26 February 2004; accepted 19 March 2004
- genetic tagging;
- microsatellite genotyping;
- noninvasive samples;
- quantitative PCR
Genetic analysis using noninvasively collected samples such as faeces continues to pose a formidable challenge because of unpredictable variation in the extent to which usable DNA is obtained. We investigated the influence of multiple variables on the quantity of DNA extracted from faecal samples from wild mountain gorillas and chimpanzees. There was a small negative correlation between temperature at time of collection and the amount of DNA obtained. Storage of samples either in RNAlater solution or dried using silica gel beads produced similar results, but significantly higher amounts of DNA were obtained using a novel protocol that combines a short period of storage in ethanol with subsequent desiccation using silica.