Advertisement

Comparison between mitochondrial and chloroplast DNA variation in the native range of Silene vulgaris

Authors

  • HELENA ŠTORCHOVÁ,

    Corresponding author
    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 165 00 Prague 6, Lysolaje, Czech Republic,
    2. Institute of Arctic Biology, University of Alaska at Fairbanks, PO Box 757000, Fairbanks, AK 99775, USA
      H. Štorchová. Present address: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 165 00 Prague 6, Lysolaje, Czech Republic. Fax: 420 220390 456; E-mail: storchova@ueb.cas.cz
    Search for more papers by this author
  • MATTHEW S. OLSON

    1. Department of Biology and Wildlife, University of Alaska at Fairbanks, Fairbanks, AK 99775, USA,
    2. Institute of Arctic Biology, University of Alaska at Fairbanks, PO Box 757000, Fairbanks, AK 99775, USA
    Search for more papers by this author

H. Štorchová. Present address: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 165 00 Prague 6, Lysolaje, Czech Republic. Fax: 420 220390 456; E-mail: storchova@ueb.cas.cz

Abstract

A detailed survey of mitochondrial and chloroplast diversity in eight populations of Silene vulgaris from Central Europe was conducted for comparison with previously published data on diversity from S. vulgaris populations in the introduced range. Mitochondrial DNA (mtDNA) variation around the coxI gene was assessed with Southern blotting/restriction fragment length polymorphism methods. Chloroplast variation was assessed by sequencing the intergenic spacer separating the trnH and psbA genes. Thirty mtDNA haplotypes and 24 chloroplast DNA (cpDNA) haplotypes were found within 86 individuals. The overall genetic diversity h (0.941 for mitochondrial, and 0.893 for chloroplast markers) and within-population diversity were higher than reported in previous population studies of S. vulgaris in the USA and Europe. The frequency of private alleles was surprisingly high — more than 90% for both kinds of markers. Most of our populations were large and located in relatively undisturbed meadows, whereas surveys in Virginia consisted of smaller roadside populations. The slow rate of population turnover in European populations is discussed as a factor responsible for the relatively high diversity of S. vulgaris in undisturbed areas of its native range. Association between mtDNA and cpDNA haplotypes was also demonstrated. Finally, gender and mtDNA haplotype were associated in the Alps populations, where females were very rare.

Ancillary