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Appendix S1 GenBank and museum voucher accession numbers for chameleons used in this study (PEM-R, Port Elizabeth Museum, South Africa). Sampling locality details are given as decimal degrees for chameleons from the Cape Floristic Region. Clade membership corresponds to Fig. 2 (clades I, IIa, IIb, IIc). K. A. Tolley field series: specimens deposited but marked ?PEM? have not yet received museum accession numbers. Appendix S2 Sequence divergences (uncorrected p distances) within and among the mtDNA lineages for Bradypodion for (a) ND2 and (b) 16S. Values on the diagonal are within-lineage sequence divergences [not calculated (n/c) for lineages with only one individual].

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