Get access
Advertisement

Tracking factors modulating cytoplasmic incompatibilities in the mosquito Culex pipiens

Authors

  • OLIVIER DURON,

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author
  • CLOTILDE BERNARD,

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author
  • SANDRA UNAL,

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author
  • ARNAUD BERTHOMIEU,

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author
  • CLAIRE BERTICAT,

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author
  • MYLÈNE WEILL

    1. Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Institut des Sciences de l"Evolution (UMR CNRS 5554), Université de Montpellier II (C.C. 065), F-34095 Montpellier, cedex 05, France
    Search for more papers by this author

Mylène Weill, Fax: (33) 4 67 14 36 22; E-mail: weill@isem.univ-montp2.fr

Abstract

Wolbachia are maternally inherited endosymbiotic bacteria that infect many arthropod species and may induce cytoplasmic incompatibility (CI), resulting in abortive embryonic development. One Wolbachia host, Culex pipiens complex mosquitoes, displays high levels of variability in both CI crossing types (cytotypes) and DNA markers. We report here an analysis of 14 mosquito strains, containing 13 Wolbachia variants, and with 13 different cytotypes. Cytotypes were Wolbachia-dependent, as antibiotic treatment rendered all strains tested compatible. Cytotype distributions were independent of geographical distance between sampling sites and host subspecies, suggesting that Wolbachia does not promote a reproductive isolation depending on these parameters. Backcross analysis demonstrated a mild restoring effect of the nuclear genome, indicating that CI is mostly cytoplasmically determined for some crosses. No correlation was found between the phenotypic and genotypic variability of 16 WO prophage and transposon markers, except for the WO prophage Gp15 gene, which encodes a protein similar to a bacterial virulence factor. However, Gp15 is partially correlated with CI expression, suggesting that it could be just linked to a CI gene.

Get access to the full text of this article

Ancillary