Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon

  1. M. BOULESTEIX1,
  2. F. SIMARD2,3,
  3. C. ANTONIO-NKONDJIO2,3,
  4. H. P. AWONO-AMBENE2,4,
  5. D. FONTENILLE3,
  6. C. BIÉMONT1

Article first published online: 15 NOV 2006

DOI: 10.1111/j.1365-294X.2006.03150.x

Issue

Molecular Ecology

Molecular Ecology

Volume 16, Issue 2, pages 441–452, January 2007

Additional Information

How to Cite

BOULESTEIX, M., SIMARD, F., ANTONIO-NKONDJIO, C., AWONO-AMBENE, H. P., FONTENILLE, D. and BIÉMONT, C. (2007), Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon. Molecular Ecology, 16: 441–452. doi: 10.1111/j.1365-294X.2006.03150.x

Author Information

  1. 1

    Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne cedex, France,

  2. 2

    Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroon,

  3. 3

    Unité de Recherche 016, Institut de Recherche pour le Développement (IRD), 911, Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France,

  4. 4

    Centre de Recherches Médicales, Institut de Recherches Médicales et des Plantes Médicinales (IMPM), BP 6163, Yaoundé, Cameroon

* Biémont Christian, Laboratoire «Biométrie et Biologie Evolutive», UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne cedex, France. Fax: (33) 4 72 43 13 88; E-mail: biemont@biomserv.univ-lyon1.fr

Publication History

  1. Issue published online: 4 JAN 2007
  2. Article first published online: 15 NOV 2006
  3. Received 5 May 2006; revision accepted 1 September 2006

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (223K)

Keywords:

  • Anopheles gambiae;
  • insertion polymorphism;
  • molecular forms;
  • population genetics;
  • speciation;
  • transposable elements

Abstract

The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations of the M and S molecular forms of the mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that the mean TE insertion site number and the within-population insertion site polymorphism globally differed between the M and S molecular forms. The comparison of the TE insertion profiles of the populations revealed a significant differentiation between these two molecular forms (0.163 < ΦST < 0.371). We cloned several insertions of a non-LTR retrotransposon (Aara8) that were fixed in one form and absent in the other one. The only insertion that could be clearly located on a chromosome arm mapped to cytological division 6 of chromosome X, confirming the importance of this region in the ongoing speciation between the M and S molecular forms.

View Full Article (HTML) Get PDF (223K)