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Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon

Authors

  • M. BOULESTEIX,

    1. Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne cedex, France,
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  • F. SIMARD,

    1. Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroon,
    2. Unité de Recherche 016, Institut de Recherche pour le Développement (IRD), 911, Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France,
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  • C. ANTONIO-NKONDJIO,

    1. Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroon,
    2. Unité de Recherche 016, Institut de Recherche pour le Développement (IRD), 911, Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France,
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  • H. P. AWONO-AMBENE,

    1. Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroon,
    2. Centre de Recherches Médicales, Institut de Recherches Médicales et des Plantes Médicinales (IMPM), BP 6163, Yaoundé, Cameroon
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  • D. FONTENILLE,

    1. Unité de Recherche 016, Institut de Recherche pour le Développement (IRD), 911, Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France,
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  • C. BIÉMONT

    1. Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne cedex, France,
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Biémont Christian, Laboratoire «Biométrie et Biologie Evolutive», UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne cedex, France. Fax: (33) 4 72 43 13 88; E-mail: biemont@biomserv.univ-lyon1.fr

Abstract

The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations of the M and S molecular forms of the mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that the mean TE insertion site number and the within-population insertion site polymorphism globally differed between the M and S molecular forms. The comparison of the TE insertion profiles of the populations revealed a significant differentiation between these two molecular forms (0.163 < ΦST < 0.371). We cloned several insertions of a non-LTR retrotransposon (Aara8) that were fixed in one form and absent in the other one. The only insertion that could be clearly located on a chromosome arm mapped to cytological division 6 of chromosome X, confirming the importance of this region in the ongoing speciation between the M and S molecular forms.

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