Genetic variation and phylogeography of free-living mouse species (genus Mus) in the Balkans and the Middle East

Authors

  • M. MACHOLÁN,

    1. Laboratory of Mammalian Evolutionary Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Veveří 97, CZ-60200 Brno, Czech Republic,
    2. Institute of Botany and Zoology, Masaryk University, Brno, Czech Republic,
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  • M. VYSKOČILOVÁ,

    1. Institute of Botany and Zoology, Masaryk University, Brno, Czech Republic,
    2. Department of Population Biology, Institute of Vertebrate Biology, ASCR, Studenec, Czech Republic,
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  • F. BONHOMME,

    1. Biologie Intégrative, Institut des Sciences de l’Evolution CNRS — Université de Montpellier 2, Montpellier Cedex 05, France,
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  • B. KRYŠTUFEK,

    1. Slovene Museum of Natural History, Ljubljana, Slovenia,
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  • A. ORTH,

    1. Biologie Intégrative, Institut des Sciences de l’Evolution CNRS — Université de Montpellier 2, Montpellier Cedex 05, France,
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  • V. VOHRALÍK

    1. Department of Zoology, Charles University, Prague, Czech Republic
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Miloš Macholán, Fax: +420–541212988; E-mail: macholan@iach.cz

Abstract

This work presents a study of the distribution and pattern of variation throughout the ranges of three free-living mouse species of the genus MusM. macedonicus, M. spicilegus, and a M. cypriacus — based on sequencing of two segments of the mitochondrial DNA (mtDNA) control region. The study shows a similar level of variability in the three species and suggests their recent population expansion. The highest proportion of variation is found within populations indicating low genetic structuring. Phylogenetic analysis confirms the significant divergence of a mitochondrial lineage of M. macedonicus from Israel, recently described as a new subspecies, M. macedonicus spretoides. Conversely, no genetic hiatus is revealed between European and Asian populations of M. macedonicus macedonicus. Although phylogenetic relationships among M. spicilegus populations could not be unravelled precisely, the results suggest a recent westward expansion of the species. The mtDNA divergence between M. macedonicus and M. spicilegus is 7.3%, suggesting their split between c. 700 000 and 1 million years ago. These dates correspond with a coalescent estimate about 720 000 years ago. On the other hand, M. cypriacus appeared almost twice as divergent from the former species (4.5%) as from the latter (8.8%) suggesting a divergence of c. 430 000–610 000 years ago (coalescent ≈ 490 000 years ago) and 830 000–1.2 million years ago (coalescent ≈ 780 000 years ago), respectively. Approximate times of population expansion have also been estimated for all taxa and groups of populations. Existence of several glacial refuges and various colonization scenarios are discussed; since all estimated divergence times fall within interglacial periods it seems that climatic oscillations did not play a crucial role in the evolution of the three species.

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