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Appendix S1 Genbank accession numbers for all previously published sequences used in the phylogeny in text Figure 3, including outgroups.

Appendix S2 A nexus format alignment of the cytochrome b sequences contributing to the phylogeny in text Figure 3.

Appendix S3 Allele frequencies for polymorphic loci, observed heterozygosity (Hobs) and heterozygosity expected under the assumption of Hardy–Weinberg equilibrium (Hexp) in each of 3 unilocular asexual gallwasp taxa.

Appendix S4 Summaries of analyses of linkage disequilibrium in the 3 unilocular asexual gallwasp taxa Andricus caputmedusae, A. glutinosus, and A. quercustozae.

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