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Fig. S1 ML trees of individual MLST genes based on 28 Wolbachia STs belonging to supergroup A. The tree was rooted with Wolbachia from the host Culex pipiens (supergroup B). For each node, ML (top) and MP (bottom) bootstrap values > 50% are shown. The 11 Agelenopsis-specific STs reported here (in bold) cluster in three separate clades (1–3) based on four out of the five genes. Recombination at gatB is responsible for the internal subdivision of clade 1 (1a and 1b).

Table S1 Screening and typing of Wolbachia strains in Agelenopsis/Barronopsis species

Table S2 Model parameters of MLST genes estimated in Modeltest

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