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Fig. S1 (a) Distribution of mean heading date without vernalization (HdNV) in the parental population, the initial population and Le Moulon, Toulouse, and Vervins populations together. (b) Distribution of mean heading date without vernalization (HdNV) in the populations of Le Moulon, Toulouse and Vervins across generations.

Fig. S2 Sequence alignment of the VRN-1A promoter amplified with genome specific primers VRN1AF/VRN1R defined in Yan et al. (2004). For the two fragments of allele vrn1A-3: (hf) = high fragment (identical to the sequence of vrn1A-2, GenBank EU127902), (lf) = low fragment (GenBank EU127903). Polymorphic regions are highlighted in gray. Boxed sections are regions putatively involved in the gene regulation as identified by Yan et al. (2003).

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