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Fig. S1 Hypotheses of phylogenetic relationships among members of the D. pulex complex, based on Bayesian inference topologies of all different partitions.

Table S1 DNA – sequences used for phylogenetic analyses, and Genbank accession number when available.

Table S2 Uncorrected pairwise genetic distances at the three sequenced mitochondrial genes between representatives of each taxon.

Table S3 Summary of phylogenetic descriptors of the different partitions and the phylogenetic results.

Table S4 Pairwise genetic distances between MLGs based on a stepwise mutation model, used to construct the minimum spanning network in Fig. 2.

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