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Table S1 Raw data used in the study. Common and scientific names for organisms and organism groups considered are given in Columns 1 and 2. The area (Column 3) refers to the geographical range of the organisms considered in each study (CARIB, Caribbean; GAL, Galápagos archipelago; MESO, Meso-America; NEO, The whole Neotropics; SA, South America). Columns 4 and 5 specify the actual DNA sequences employed (mt/chl, mitochondrial and chloroplastic; nuc, nuclear). GDR (Column 6) is the estimated genetic divergence rate in percentage per Myr. Columns 7 and 8 express the time range in Myr for the origin of the taxa considered, as represented by the age of the older (Column 7) and the younger (Column 8) ESU of the lineage. The corresponding geological age is given in Column 9, using the 2.6 Myr boundary for the beginning of the Pleistocene (Eo, Eocene; Mio, Miocene’ Plio, Pliocene; Ple, Pleistocene; E, Early; L, Late). Taxa (Column 10) refers to the nature of the lower significant taxonomic category (Evolutionarily Significant Unit, ESU) considered in each study (S, species; SP, subspecies; GC, geographical clades). Column 11 (total) indicates the number of ESUs employed in each study. In the case of studies covering a wide geographical range, only neotropical ESUs were considered. Columns 12 and 13 give the number of ESUs originated either in preglacial (pG) or glacial (G) times (see text for details). When raw data from the original references are not sufficient to estimate these proportions, pG and G cells are blank. The sum of these two columns does not necessarily coincide with the total of Column 11, as not always has been possible to estimate the age of divergence of all the ESUs in a group. Reference numbers are given in Column 14

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