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Fig. S1 Interleaved alignment of four-loop conotoxin transcript sequences of Conus abbreviatus, C. aristophanes, C. coronatus, C. ebraeus, C. judaeus and C. miliaris. Loci are identified as follows: A, C. abbreviatus; Ar, C. aristophanes; C, C. coronatus; E, C. ebraeus; J, C. judaeus; and M, C. miliaris; arbitrary numerical identifiers are given for each putative locus. Sequences are arranged according to their position in the tree (Fig. 2). Regions of the transcripts are colour coded as follows: yellow, prepro coding region; red/turquoise, toxin coding region (cysteine codons are red); stop codon, dark yellow; 3′ untranslated region, gray.

Fig. S2 Interleaved alignment of predicted amino acid sequences of four-loop conotoxin transcript sequences of Conus abbreviatus, C. aristophanes, C. coronatus, C. ebraeus, C. judaeus and C. miliaris. Loci are identified as in Fig. S1. Sequences are arranged according to their position in the tree (Fig. 2). Regions of the peptide are colour coded as follows: yellow, prepro coding region; red/turquoise, toxin coding region (cysteine codons are red); stop codon, dark yellow. Amino acids following the terminal cysteine residue of the toxin coding region are given as they occur and are not based on alignments of nucleotide sequences because of the presence of insertions/deletions that affect reading frame (see Fig. S1).

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