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Fig. S1 Photographs of (A) Ctenosaura hemilopha (B) C. hemilopha × C. pectinata hybrid and (C) C. pectinata male individuals, highlighting the intermediate morphological features of the hybrid. Note the enlarged but not extended dorsal crest (a) in the hybrid; the spotted colouration pattern exhibiting clear bands around the body (b); and the black colouration on the gular and pectoral region (c) in comparison to the pure individuals.

Fig. S2 Majority-rule consensus tree of 1802 trees sampled during two independent runs of a Bayesian analysis implementing a lognormal relaxed molecular clock. Assigned ages for external calibration points (black circle and square) are denoted in italics and correspond to fossil records of Pristiguana (93 Ma) and Dipsosaurus (24 Ma). Estimated 95% confidence age intervals and posterior probabilities for nodes of interest are shown under branch. Gene bank accession numbers are indicated in Table S1.

Table S1 GenBank accession numbers of taxa included in the Bayesian analysis for estimation of divergence times

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