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Fig. S1 Histograms showing the frequency distribution of microsatellite alleles in Leopardus tigrinus (grey bars), L. geoffroyi (black bars) and L. colocolo (white bars). Each graph depicts alleles from a single microsatellite locus, identified at the top.

Fig. S2 Mismatch distribution analysis of the Leopardus tigrinus and L. geoffroyi clades, based on concatenated mtDNA control region and ND5 sequences (795 bp, excluding all sites with missing information or gaps). The dashed line represents the observed pattern, while the continuous line depicts the pattern expected under a model of sudden demographic expansion.

Table S1 Samples of Neotropical felids analyzed in the present study

Table S2 Bayesian clustering analyses performed with STRUCTURE using the correlated frequencies model and no phenotype-based information. Each value corresponds to the average among five different runs

Table S3 Population assignment of Leopardus tigrinus and L. geoffroyi individuals using a STRUCTURE analysis with no phenotype-based information. The membership proportion for each individual is expressed as a q-value followed by its respective credibility interval in parentheses. Individuals with probabilities ≤ 0.90 of belonging to their phenotype-based species are shown in bold. Abbreviations for individual ID and geographical origin are the same as used in Figure 1 and Table S1, Supplementary material.

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