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Fig. S1 Neighbour-joining tree of 88 cytochrome b haplotypes and their locations. NA, North America; BS, Black Sea; AS, Azov Sea; VR, Volga River; CS, Caspian Sea. Bootstrap values (1000 replicates) are given as percentages on nodes.

Fig. S2 Neighbour-joining trees of round goby population samples based on Cavalli-Sforza & Edwards (1967) chord distances, in PHYLIP version 3.6 (Felsenstein 1989, 2008). Bootstrap values (1000 replicates) are given on nodes. *Eurasian source locations (Brown & Stepien 2008). A. Distances based on microsatellite data. B. Distances based on cytochrome b data, showing secondary (expansion) founder effects.

Table S1 Occurrence of mtDNA cytochrome b haplotypes in North American round goby samples (lettered according to Fig. 1, with GenBank (http://www.ncbi.nlm.nih.gov) Accession numbers and frequency. Eurasian frequencies of haplotypes present in North American round goby samples included for comparison. *identified in Eurasia by Brown & Stepien (2008). The Sea of Azov, Volga River, and Caspian Sea locations share no haplotypes with North America.

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