S. Creer and V. G. Fonseca are joint first authors of this work.
Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises
Article first published online: 10 FEB 2010
© 2010 Blackwell Publishing Ltd
Special Issue: Next Generation Molecular Ecology
Volume 19, Issue Supplement s1, pages 4–20, March 2010
How to Cite
CREER, S., FONSECA, V. G., PORAZINSKA, D. L., GIBLIN-DAVIS, R. M., SUNG, W., POWER, D. M., PACKER, M., CARVALHO, G. R., BLAXTER, M. L., LAMBSHEAD, P. J. D. and THOMAS, W. K. (2010), Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises. Molecular Ecology, 19: 4–20. doi: 10.1111/j.1365-294X.2009.04473.x
SC is a molecular ecologist with broad interests in the spheres of ecology and evolution. Current research foci include understanding the mechanisms underpinning the role, origins and dynamics of biodiversity in ecosystem processes, environmental genomics, molecular phylogenetics and population genetics. VGF’s research interests focus on functional and environmental genomic aspects of differential gene expression under normal and pathological conditions, the analysis of marine biodiversity from a taxonomic and evolutionary perspective, and ecology and management of marine ecosystems. DP and RGD are interested in the patterns of terrestrial microbiotic diversity and their role in ecosystem functioning using ultrasequencing approaches as well as in molecular phylogeny of terrestrial and entomophilic nematodes for linking metagenetics with traditional taxonomy. DMP is interested in molecular evolution traced using gene families, particularly those regulating whole animal homeostasis. MP is an ecologist specializing in marine benthic biodiversity. PJD is a nematologist interested in the quantitative analysis of biodiversity and its origins. GRC’s research is aimed at the elucidation of fundamental aspects of a species’ biology such as patterns of dispersal and gene flow, evolution of life histories and behaviour, response to environmental stress, and mechanisms of speciation. MB is a genomics biologist, with major interests in the genomics and molecular identification of “neglected” animal phyla. WKT and WS are interested in patterns and processes of genomic change and how these relate to our understanding of genetic diversity and evolution, in addition to the analysis and interpretation of metagenetic data.
- Issue published online: 10 FEB 2010
- Article first published online: 10 FEB 2010
- Received 19 June 2009; revision received 19 August 2009; accepted 21 August 2009
Data 1. List of potential barcode tag primer keys currently used for environmental metagenetic work from the Josephine Bay Paul Center’s Visualization and Analysis of Microbial Population Structures (VAMPS) web resource (http://vamps.mbl.edu/resources/keys.php).
Data 2. Per cent similarity matches for the studied primers were deduced using up to 13 representative sequences from each Metazoan taxon containing meiofaunal representatives from GenBank sequence database for nSSU sequences.
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.
|MEC_4473_sm_Supplementarymaterial.doc||27K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.