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Table S1 Dicamptodon aterrimus sampling reaches.  Map datum WGS84 was used for GPS coordinates. Fifteen individuals were sampled from throughout the length of each survey reach, with the exception of RBU1 where 16 individuals were sampled. Sampling streams are mapped in Fig. 3

Table S2 Microsatellite loci used to genotype Dicamptodon aterrimus (Curtis and Taylor 2000; Steele et al. 2008). Primer sequences are given with fluorescent marker applied to forward primers, including additional base pairs added as “pig tails” where required. Repeat units of microsatellites are listed, NA is the number of alleles per locus, length refers to the size range of products, and TA is the annealing temperature used for PCR amplification. Temperature ranges are given for touchdown profiles used to amplify multiplexes or single PCRs

Table S3 Genetic diversity of each stream where AS is allelic richness, NA is the total number of alleles observed in the stream, HO is observed heterozygosity, HE is expected heterozygosity, and FIS is provided for all 9 loci when polymorphic. FIS values that are significantly different from zero are in bold, as well as the two streams with significant departures from Hardy-Weinberg (HW) proportions. After correcting for multiple tests, however, none of the FIS values were significantly different from zero, and no populations had significant deviations from HW proportions. Fifteen individuals were genotyped in each stream with the exception of RBU1 with 16 individuals

Table S4 Pairwise FST among all streams. Values that are not significantly different from zero are in bold.  Pairs of streams within the same catchment are highlighted in grey. Significance testing of FST was based on 10,000 permutations

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