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Fig. S1 Location of 15 of 1000 simulated random straight lines that bisect the Cotswolds study area.

Fig. S2 Geographic distances between all pairs of individuals in area A, plotted against pairwise genetic kinship between individuals (estimated using Loiselle’s Fij) for (a) pairs of individuals on the same side of the river Wye and pairs of individuals (b) on separate sides of the river. In the within-group comparison, the grey line represents the slope of the isolation-by-distance pattern considering all individuals, while the black line represents the same slope, but calculated over the same range of geographic distances as obtained in the among-group comparison.

Fig. S3 Geographic distance between related pairs on individuals in area A plotted against pairwise relatedness, calculated according to Queller & Goodnight (1989). Choice of related individuals is based on the simulation of 10 000 unrelated individuals (see Materials and methods). All pairs were located on the same side of the river Wye.

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