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Fig. S1 Replication of CHIKV E1-226V in BHK21 cells and C6/36 cells. Cells were infected at a MOI of 0.1 PFU/cell. After 1h of absorption, inoculum was removed and supernatant was harvested at different hours post-infection (pi) and titrated on Vero cells to estimate the number of PFU/mL. The viral stock was harvested at 48h pi.

Fig. S2 Replication of CHIKV E1-226V in C6/36 cells. Cells were infected at a MOI of 0.1 PFU/cell. After 1h of absorption, inoculum was removed and supernatant was harvested at different hours post-infection. Then, supernatants were titrated on Vero cells to estimate the number of PFU/mL and by quantitative RT-PCR to evaluate the number of viral RNA copies/mL. The number of RNA copies was between 1 and 2 log higher than the number of PFU.

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