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Fig. S1 (a) Growth profiles of the 26 original clones without (in black), and with (in red) the arcA mutation. The numbers indicate the generations. SS growth profiles are indicated in the upper part of the figure, FS profiles in the lower part. For each growth profile, thick lines indicate the average growth profiles, thin lines indicate the standard error of the mean, the x axis indicates time since inoculation and the y axis indicates the optical density (600nm). A * indicates the presence of the mutation in the original clone. (b) Frequency dependence between FS and SS. Each graph indicates the result of competition experiments, initiated at two different frequencies, between one FS and one SS clone isoalted at the same time point during the evolution experiment. The x-axis indicates the number of days of competition, the y-axis the frequency of FS clones. SE are indicated.

Fig. S2 Results of the competition experiments, initiated with two different frequencies, between strains isogenic except for the arcA mutation (a) in SS clones and (b) in FS clones. Each graph corresponds to one clone isolated during the evolution experiment. The x-axis indicates the days of competition (each mark corresponds to 2 days). The y-axis indicates the frequency of the mutation (each mark corresponds to 0.2). SE are indicated.

Fig. S3 Results of competition experiments, initiated with two different frequencies, between strains isogenic except for the arcA mutation (left column) and between these same strains plus a clone from the other ecotype isolated at the same time point during the experimental evolution (right column) (a) in SS clones and (b) in FS clones. The x-axis indicates the days of competition (each mark corresponds to 2 days). The y-axis indicates the frequency of the mutation (each mark corresponds to 0.2). SE are indicated.

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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.