Comparative landscape genetics of two pond-breeding amphibian species in a highly modified agricultural landscape
Version of Record online: 13 AUG 2010
© 2010 Blackwell Publishing Ltd
Special Issue: SPECIAL ISSUE ON LANDSCAPE GENETICS
Volume 19, Issue 17, pages 3650–3663, September 2010
How to Cite
GOLDBERG, C. S. and WAITS, L. P. (2010), Comparative landscape genetics of two pond-breeding amphibian species in a highly modified agricultural landscape. Molecular Ecology, 19: 3650–3663. doi: 10.1111/j.1365-294X.2010.04673.x
- Issue online: 25 AUG 2010
- Version of Record online: 13 AUG 2010
- Received 20 November 2009; revision received 5 April 2010; accepted 12 April 2010
Table S1 PCR conditions for amplifying microsatellites in (A) Columbia spotted frogs and (B) long-toed salamanders. PCR profile was: initial denaturing 95 °C for 15 min, followed by 35–38 cycles of 95 °C for 30 s, annealing temperature for 90 s, and 72 °C for 60 s, followed by final extension of 60 °C for 30 min. Touchdowns were staged in increments of 0.5 °C. All reactions used 1X Qiagen Multiplex Master Mix. Q indicates 5X Qiagen Q solution
Fig. S1 Population ancestry values derived from STRUCTURE analyses (Prichard et al. 2000), indicating the most probable number of genetic groups using the ΔK statistic. Individual-based measurements yield very similar results. We conducted 10 simulations for each number of distinct genetic groups (K), ranging from 1 to 10, used the admixture model, and assumed correlated allele frequencies (Falush et al. 2003). The burn-in length was 100 000 and the run length was 500 000. We used the ΔK statistic (Evanno et al. 2005) to determine the most likely number of groups, and examined ancestry values for each individual and population graphically.
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