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Table S1: genotypes of all queencell content (QCC). For QCC homozygous at one or more loci for a queen allele, we calculated the probability that this is due to the queen having mated with a drone carrying the same allele as the queen at that locus. When no queenbrood was present, this was not calculated. ProbabiliBes in red denote the probabiliBy of this QCC being produced by a non-natal instead of a natal worker (see text). The origin of foreign laid (FL) QCC are given when known. ‘No queen eggs’: no more queen eggs present in the colony at the Bme the QCC was produced. Colony A1-A7: colonies that were spaced apart; colonies LV1-LV8: colonies that were placed close together.

Table S2 Populaton allele frequencies.

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