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Fig. S1. The most common life-cycle pattern among diplostomoid trematodes.

Fig. S2 Diversity of diplostomoid metacercariae in fish from the St. Lawrence River detected by DNA barcoding compared to previous Canadian records.

Fig. S3. Species accumulation curves for diplostomoids from six focal fish species.

Fig. S4 Phylogeny used to generate independent contrasts.

Table S1 Summary of previous molecular studies of diplostomoids.

Table S2 Diplostomoid species detected by DNA barcoding.

Table S3 Distribution of Ornithodiplostomum sp. 1 across localities and host species in the St. Lawrence River.

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