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Appendix S1 Design matrix of local adaptation experiment. Cell contain the number of clones for each water by population source combination. Note that clones were nested within populations for the statistical analysis. While the target sample size was 8 for each treatment, some clonal mothers did not produce enough neonates during the experimental setup to be included in the experiment. Only two replicates were lost due to experimental deaths (one from Busey (Pop.) × Top (Res.) and one from Dump × Dump).

Appendix S2 Test for isolation by distance in genetic differentiation among populations. The correlation between genetic and geographic distance was high, but not significant (Mantel r = 0.84, P = 0.17), likely due to the low number of populations in the sample (n = 4).

Appendix S3 Individual assignments among four clusters using the program Structure. Each bar represents one individual and is broken into four colors whose heights correspond to the proportion of times the individual was assigned to a particular cluster. Individuals are grouped by their pond of origin for clarity. The program was run for 100 000 burn in replications and 500 000 MCMC replications to calculate cluster assignments.

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