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Fig. S1 (A) Results of Bayesian cluster analysis using the program STRUCTURE showing log probability of the data Ln Pr(X|K) as a function of number of putative populations (K). (B) The values of Δ(K) calculated according to Evanno et al. (2005) that were used for selection of the best fitted model.

Fig. S2 (A) Frequency of the allele 228 at locus Rser04 (in blue). (B) Frequency of allele 126 at locus Rser13 (in blue).

Fig. S3 Comparison of the relative posterior probabilities for all scenarios separately for each of four steps. They are computed via the logistic regression in DIYABC 0.8.1 (see details in Cornuet et al. 2008) on 1% of simulated datasets closest to the observed data.

Fig. S4 Schemes of the best scenarios for each of the four steps. Effective population sizes are marked in different colours and in the left, times of events (not to scale) are in the right. For the population codes see Table 3, and for the parameter codes see Supplemetary Tables 3-6.

Table S1 Summary of genotyped microsatellite loci

Table S2 Pairwise Fst estimates between sampled populations

Table S3 The prior distributions of parameters used for description of the 32 scenarios analysed via the Approximate Bayesian Computation (ABC, Beaumont et al. 2002) in DIYABC 0.8.1 (Cornuet et al. 2008)

Table S4 Estimations of the posterior distributions of parameters revealed from the Approximate Bayesian Computation (ABC, Beaumont et al. 2002) for the best scenario for the first step (scenario 1.1 in Table 3)

Table S5 Estimations of the posterior distributions of parameters revealed from the Approximate Bayesian Computation (ABC, Beaumont et al. 2002) for the best scenario for the second step (scenario 2.1 in Table 3)

Table S6 Estimations of the posterior distributions of parameters revealed from the Approximate Bayesian Computation (ABC, Beaumont et al. 2002) for the best scenario for the third step (scenario 3.1 in Table 3)

Table S7 Estimations of the posterior distributions of parameters revealed from the Approximate Bayesian Computation (ABC, Beaumont et al. 2002) for the two best scenarios for the fourth step (scenarios 4.3 and 4.1 in Table 3)

Table S8 Haplotypes of cytochrome b (425 bp) analysed in this study

Table S9 Distribution of haplotypes of partial cytochrome b (425 bp) at localities under study. *Bohlen et al. (2006) indicated the corresponding haplotypes. ** For abbreviation of populations – see Table 1

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MEC_4844_sm_FigsS1-S4.ppt1262KSupporting info item
MEC_4844_sm_TableS9.xls93KSupporting info item
MEC_4844_sm_TablesS1-S7.doc327KSupporting info item
MEC_4844_sm_TablesS8-S9.xls94KSupporting info item

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