Phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding: the case of the Tricholoma scalpturatum complex (Basidiomycota)

Authors

  • PATRICIA JARGEAT,

    1. Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France
    2. CNRS, UMR5174 EDB, F-31062 Toulouse, France
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    • These authors have equally contributed to this work.

  • FLORENT MARTOS,

    1. Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France
    2. CNRS, UMR5174 EDB, F-31062 Toulouse, France
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    • These authors have equally contributed to this work.

    • Present address: Université de La Réunion, UMR C53. Peuplements Végétaux et Bioagresseurs en Milieu Tropical, 15 Av. R. Cassin, BP 7151, F-97715 Saint Denis Cedex 9, France.

  • FABIAN CARRICONDE,

    1. Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France
    2. CNRS, UMR5174 EDB, F-31062 Toulouse, France
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    • Present address: Molecular Mycology Research Laboratory, CIDM, ICPMR, Westmead Hospital, Darcy Road, Westmead, NSW 2145, Australia.

  • HERVÉ GRYTA,

    1. Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France
    2. CNRS, UMR5174 EDB, F-31062 Toulouse, France
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  • PIERRE-ARTHUR MOREAU,

    1. Faculté des Sciences Biologiques et Pharmaceutiques, Département de Botanique, BP83, F-59006 Lille, France
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  • MONIQUE GARDES

    1. Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France
    2. CNRS, UMR5174 EDB, F-31062 Toulouse, France
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Dr Patricia Jargeat, Fax: +33 561 55 73 27; E-mail jargeat@cict.fr

Abstract

Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.

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