These authors contributed equally to this work.
Genomic-scale capture and sequencing of endogenous DNA from feces
Article first published online: 3 NOV 2010
© 2010 Blackwell Publishing Ltd
Volume 19, Issue 24, pages 5332–5344, December 2010
How to Cite
PERRY, G. H., MARIONI, J. C., MELSTED, P. and GILAD, Y. (2010), Genomic-scale capture and sequencing of endogenous DNA from feces. Molecular Ecology, 19: 5332–5344. doi: 10.1111/j.1365-294X.2010.04888.x
Data deposition: All sequence data have been deposited at the National Center for Biotechnology Information short read archive (http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi) as study no. SRA012374.
- Issue published online: 6 DEC 2010
- Article first published online: 3 NOV 2010
- Received 22 June 2010; revision received 1 September 2010; accepted 7 September 2010
Fig. S1 Heterozygous site single nucleotide polymorphism identification criteria.
Fig. S2 Sequence coverage correspondence.
Fig. S3 Validation of identified single nucleotide polymorphisms by PCR and Sanger sequencing.
Table S1 Sequencing statistics summary
Table S2 PCR amplification and sequencing primers
Table S3 Sample-level heterozygosity, for sites with coverage sufficient for SNP identification from both fecal and blood DNA results for each individual
The following Datasets (S1 and S2) are available from http://giladlab.uchicago.edu/data/datasetS1/
Datasets S1 Filtered per-site nucleotide sequence coverage.
Datasets S2 Genotypes for identified single nucleotide polymorphisms.
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