Present address: Institut du Littoral et de l’Environnement, UMR LIENSs 6250, CNRS-Université de la Rochelle, 2 rue Olympes de Gouges, 17000 LA ROCHELLE France.
Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing
Article first published online: 9 DEC 2010
© 2010 Blackwell Publishing Ltd
Volume 20, Issue 2, pages 258–274, January 2011
How to Cite
AGOGUÉ, H., LAMY, D., NEAL, P. R., SOGIN, M. L. and HERNDL, G. J. (2011), Water mass-specificity of bacterial communities in the North Atlantic revealed by massively parallel sequencing. Molecular Ecology, 20: 258–274. doi: 10.1111/j.1365-294X.2010.04932.x
- Issue published online: 6 JAN 2011
- Article first published online: 9 DEC 2010
- Received 20 January 2010; revision received 30 September 2010; accepted 6 October 2010
Table S1 Description of the 45 samples from the North Atlantic Ocean selected for bacterial Pyrosequencing
Table S2 Water mass properties and number of samples (n)
Table S3 ANOSIM test between water masses based on the relative abundance of all OTUs (97%) except the singletons
Table S4 Characteristics of the 100 most abundant OTUs
Fig. S1 Temperature – salinity diagram based on all the samples.
Fig. S2 (A) Rarefaction curves for subsurface (≅100 m depth) samples. (B) Rarefaction curves for mesopelagic samples. (C) Rarefaction curves for bathypelagic samples.
Fig. S3 Evenness (Gini index) for each bacterial OTUs (97%) except the singletons from (A) subsurface, (B) mesopelagic and (C) bathypelagic samples vs. the rank (based on the relative abundance) of that OTU. A Gini index of 1 would indicate a very uneven OTU whereas a Gini index of 0 would indicate a perfectly even OTU.
Fig. S4 Non-metric multidimensional analysis based on relative abundance of (A) all pyrotags except the singletons, (B) abundant pyrotags (frequency >1% within a sample) and (C) rare pyrotags (frequency <0.1% within a sample). Individual samples were affiliated to their respective water-mass. Superimposed circles represent clusters of samples at similarity values of (A) 30 and 40%, (B) 60 and 80% and (C) 20 and 30% (Bray-Curtis similarity). The final solution was based on 25 iterations with a final stress of (A) 0.10 and (B) 0.13.
Fig. S5 Percent of similarity (calculated through SIMPER analysis) between depth zones.
Data S1 SIMPER analysis – Contribution (Contrib%) of the OTUs to the differencesbetween water masses (Av.Abund., average abundance of each OTU per water mass; Cum.%, cumulative contribution).
|MEC_4932_sm_DatasetS1.xls||152K||Supporting info item|
|MEC_4932_sm_SI_Methods-Tables-Figures.pdf||4146K||Supporting info item|
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