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Table S1 Description of the 45 samples from the North Atlantic Ocean selected for bacterial Pyrosequencing

Table S2 Water mass properties and number of samples (n)

Table S3 ANOSIM test between water masses based on the relative abundance of all OTUs (97%) except the singletons

Table S4 Characteristics of the 100 most abundant OTUs

Fig. S1 Temperature – salinity diagram based on all the samples.

Fig. S2 (A) Rarefaction curves for subsurface (≅100 m depth) samples. (B) Rarefaction curves for mesopelagic samples. (C) Rarefaction curves for bathypelagic samples.

Fig. S3 Evenness (Gini index) for each bacterial OTUs (97%) except the singletons from (A) subsurface, (B) mesopelagic and (C) bathypelagic samples vs. the rank (based on the relative abundance) of that OTU. A Gini index of 1 would indicate a very uneven OTU whereas a Gini index of 0 would indicate a perfectly even OTU.

Fig. S4 Non-metric multidimensional analysis based on relative abundance of (A) all pyrotags except the singletons, (B) abundant pyrotags (frequency >1% within a sample) and (C) rare pyrotags (frequency <0.1% within a sample). Individual samples were affiliated to their respective water-mass. Superimposed circles represent clusters of samples at similarity values of (A) 30 and 40%, (B) 60 and 80% and (C) 20 and 30% (Bray-Curtis similarity). The final solution was based on 25 iterations with a final stress of (A) 0.10 and (B) 0.13.

Fig. S5 Percent of similarity (calculated through SIMPER analysis) between depth zones.

Data S1 SIMPER analysis – Contribution (Contrib%) of the OTUs to the differencesbetween water masses (Av.Abund., average abundance of each OTU per water mass; Cum.%, cumulative contribution).

FilenameFormatSizeDescription
MEC_4932_sm_DatasetS1.xls152KSupporting info item
MEC_4932_sm_SI_Methods-Tables-Figures.pdf4146KSupporting info item

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