Looking at both sides of the invasion: patterns of colonization in the violet tunicate Botrylloides violaceus
Article first published online: 24 DEC 2010
© 2010 Blackwell Publishing Ltd
Volume 20, Issue 3, pages 503–516, February 2011
How to Cite
BOCK, D. G., ZHAN, A., LEJEUSNE, C., MacISAAC, H. J. and CRISTESCU, M. E. (2011), Looking at both sides of the invasion: patterns of colonization in the violet tunicate Botrylloides violaceus. Molecular Ecology, 20: 503–516. doi: 10.1111/j.1365-294X.2010.04971.x
- Issue published online: 17 JAN 2011
- Article first published online: 24 DEC 2010
- Received 8 April 2010; revision received 17 November 2010; accepted 23 November 2010
Appendix S1 Occurrence of Botrylloides violaceus mtDNA COI haplotypes for in 25 North American locations, with GenBank accession numbers and frequency.
Appendix S2 Neighbor-joining phylogenetic reconstruction of Botrylloides violaceus cytochrome c oxidase subunit I (COI) haplotypes. Numbers at phylogenetic nodes indicate the neighbor-joining and maximum-likelihood bootstrap support with 1,000 replicates. The number of samples possessing each haplotype is presented in brackets.
Appendix S3 Pairwise ФST comparisons for Botrylloides violaceus populations using the mitochondrial COI marker. *Significant (P < 0.05); ** remains significant after sequential Bonferroni correction (Rice 1989). Populations on the West coast are separated by a box in the upper left corner.
Appendix S4 Clonal genotypes observed in the dataset with N, number of times the genotype appears in the dataset; Ngen, number of genotypes in the population of origin; Psib, probability of identity considering strict sibs reproduction; PHW, probability of identity under Hardy-Weinberg equilibrium. Clonal genotypes shared between locations are indicated with an asterisk.
Appendix S5 Microsatellite allele frequency for Botrylloides violaceus from 25 locations in the North American invaded range.
Appendix S6 Genetic diversity at 13 microsatellite loci for 23 sites of the violet tunicate, Botrylloides violaceus. N, sample size; NA, number of alleles; A, allele richness; HO, observed heterozygosity; HE, expected heterozygosity; FIS value; PHW, exact P-value for Hardy-Weinberg equilibrium. Significant departures from equilibrium after sequential Bonferroni correction are indicated in bold.
Appendix S7 Pairwise FST comparisons for Botrylloides violaceus populations using 13 microsatellite markers. *Significant (P < 0.05); ** remains significant after sequential Bonferroni correction (Rice 1989). Populations on the West coast are separated by a box in the upper left corner. Populations with sample size of less than 15 individuals (N < 15) were not included in this analysis.
Appendix S8 The log probability of the data, LnP (X| K) ± SD, and the rate of change in the probability between successive runs (_K), as a function of K for the 25 Botrylloides violaceus populations.
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