Present address: The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin EH25 9PS, UK.
Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs
Article first published online: 12 JAN 2011
© 2011 Blackwell Publishing Ltd
Special Issue: FRONTIERS IN HOST-PARASITE ECOLOGY AND EVOLUTION
Volume 20, Issue 5, pages 910–919, March 2011
How to Cite
PEMBERTON, J. M., BERALDI, D., CRAIG, B. H. and HOPKINS, J. (2011), Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs. Molecular Ecology, 20: 910–919. doi: 10.1111/j.1365-294X.2010.04992.x
- Issue published online: 16 FEB 2011
- Article first published online: 12 JAN 2011
- Received 5 August 2010; revision received 6 December 2010; accepted 9 December 2010
- disease biology;
Digital gene expression (DGE) analysis offers a route to gene discovery which by-passes the need to develop bespoke arrays for nonmodel species, and is therefore a potentially valuable tool for molecular ecologists. Scottish blackface sheep, which vary in resistance to the common abomasal parasitic nematode Teladorsagia circumcincta, were trickle-infected with L3 larvae over 3 months to mimic the natural progression of infection. DGE was performed on abomasal lymph node tissue after the resolution of infection in resistant animals. Susceptible (low resistance) animals showed a large number of differentially expressed genes associated with inflammation and cell activation, but generally few differentially regulated genes in either the susceptible or the resistant group were directly involved in the adaptive immune function. Our results are consistent with the hypothesis that both resistance and susceptibility are active responses to infection and that susceptibility is associated with dysfunction in T cell differentiation and regulation.