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Table S1 Site, location, GPS coordinates and sample size (n) for 31 baseline round goby, Neogobius melanostomus, lake populations from Lake Erie, Ontario, and Huron used as reference populations for genotype assignment of river migrants (Dufour 2007).

Table S2 Pairwise comparisons of Weir and Cockerham’s (1984) FST among round goby, Neogobius melanostomus, sampling sites at the Maitland (2005–2009), Grand (2008–2009), and Saugeen (2009) rivers.

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