These authors contributed equally to this study.
Scanning the European corn borer (Ostrinia spp.) genome for adaptive divergence between host-affiliated sibling species
Article first published online: 7 MAR 2011
© 2011 Blackwell Publishing Ltd
Volume 20, Issue 7, pages 1414–1430, April 2011
How to Cite
MIDAMEGBE, A., VITALIS, R., MALAUSA, T., DELAVA, É., CROS-ARTEIL, S. and STREIFF, R. (2011), Scanning the European corn borer (Ostrinia spp.) genome for adaptive divergence between host-affiliated sibling species. Molecular Ecology, 20: 1414–1430. doi: 10.1111/j.1365-294X.2011.05035.x
- Issue published online: 22 MAR 2011
- Article first published online: 7 MAR 2011
- Received 19 October 2010; revision revised 13 December 2010; accepted 16 December 2010
- adaptive divergence;
- host-plant specialization;
- Ostrinia spp.;
- population genomics
It has recently been shown that the European corn borer, a major pest of maize crops, is actually composed of two genetically differentiated and reproductively isolated taxa, which are found in sympatry over a wide geographical range in Eurasia. Each taxon is adapted to specific host plants: Ostrinia nubilalis feeds mainly on maize, while O. scapulalis feeds mainly on hop or mugwort. Here, we present a genome scan approach as a first step towards an integrated molecular analysis of the adaptive genomic divergence between O. nubilalis and O. scapulalis. We analysed 609 AFLP marker loci in replicate samples of sympatric populations of Ostrinia spp. collected on maize, hop and mugwort, in France. Using two genome scan methods based on the analysis of population differentiation, we found a set of 28 outlier loci that departed from the neutral expectation in one or the other method (of which a subset of 14 loci were common to both methods), which showed a significantly increased differentiation between O. nubilalis and O. scapulalis, when compared to the rest of the genome. A subset of 12 outlier loci were sequenced, of which 7 were successfully re-amplified as target candidate loci. Three of these showed homology with annotated lepidopteran sequences from public nucleotide databases.