Reinforcement selection acting on the European house mouse hybrid zone

Authors

  • BARBORA VOŠLAJEROVÁ BÍMOVÁ,

    1. Department of Population Biology, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    2. Laboratory of Mammalian Evolutionary Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Veveří 97, CZ-60200 Brno, Czech Republic
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  • MILOŠ MACHOLÁN,

    1. Laboratory of Mammalian Evolutionary Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Veveří 97, CZ-60200 Brno, Czech Republic
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  • STUART J. E. BAIRD,

    1. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, Vairão, Portugal
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  • PAVEL MUNCLINGER,

    1. Biodiversity Research Group, Department of Zoology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
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  • PETRA DUFKOVÁ,

    1. Department of Population Biology, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    2. Department of Genetics, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic
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  • CHRISTINA M. LAUKAITIS,

    1. College of Medicine, University of Arizona, Tucson, AZ, USA
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  • ROBERT C. KARN,

    1. College of Medicine, University of Arizona, Tucson, AZ, USA
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  • KENNETH LUZYNSKI,

    1. Department of Ecology and Evolutionary Biology and Museum of Zoology, University of Michigan, Ann Arbor, MI, USA
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  • PRISCILLA K. TUCKER,

    1. Department of Ecology and Evolutionary Biology and Museum of Zoology, University of Michigan, Ann Arbor, MI, USA
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  • JAROSLAV PIÁLEK

    1. Department of Population Biology, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic
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    • Present address: Department of Population Biology, IVB, ASCR, Studenec, Czech Republic.


Miloš Macholán, Fax: +420 541212988;
E-mail: macholan@iach.cz

Abstract

Behavioural isolation may lead to complete speciation when partial postzygotic isolation acts in the presence of divergent-specific mate-recognition systems. These conditions exist where Mus musculus musculus and M. m. domesticus come into contact and hybridize. We studied two mate-recognition signal systems, based on urinary and salivary proteins, across a Central European portion of the mouse hybrid zone. Introgression of the genomic regions responsible for these signals: the major urinary proteins (MUPs) and androgen binding proteins (ABPs), respectively, was compared to introgression at loci assumed to be nearly neutral and those under selection against hybridization. The preference of individuals taken from across the zone regarding these signals was measured in Y mazes, and we develop a model for the analysis of the transition of such traits under reinforcement selection. The strongest assortative preferences were found in males for urine and females for ABP. Clinal analyses confirm nearly neutral introgression of an Abp locus and two loci closely linked to the Abp gene cluster, whereas two markers flanking the Mup gene region reveal unexpected introgression. Geographic change in the preference traits matches our reinforcement selection model significantly better than standard cline models. Our study confirms that behavioural barriers are important components of reproductive isolation between the house mouse subspecies.

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