Table S1 Primer sequences and characteristics of plastid DNA microsatellite loci in Pitcairnia spp., including locus name, plastid DNA region, primer sequences, repeat type, allele size range, and no. of alleles.

Table S2 Genetic variability at 15 microsatellite loci in P.  staminea, P.  albiflos, and their hybrids, including locus name, number of alleles (A), observed heterozygosity (HO), expected heterozygosity (HE), standardized measure of differentiation (GST), fixation index (FST), total inbreeding coefficient (FIT), and within-population inbreeding coefficient (FIS).

Table S3 Analysis of Molecular Variance (amova) based on nuclear microsatellite data for P.  staminea and P.  albiflos for two different hierarchical models. (A) species level. (B) geographical level (inland vs. coastal inselbergs). Percentages of variation in parentheses refer to models in which hybrids were nested with P.  albiflos.

Table S4 Genetic divergence (FST; below diagonal) and gene flow (Nem; above diagonal) for pairs of populations of the inselberg bromeliads Pitcairnia albiflos and P.  staminea and their hybrids. Nem = (1/FST − 1)/4.

Table S5 Locus-specific, bidirectional introgression rates between Pitcairnia albiflos and P.  staminea on PAO population.

Fig. S1 Magnitude of ΔK from structure analysis as a function of K (mean ± SD over 10 replicates), calculated following the ΔK method proposed by Evanno et al. (2005), for Pitcairnia albiflos and P.  staminea microsatellite data.

Fig. S2 Posterior probabilities (q) of Pitcairnia albiflos and Pitcairnia staminea for the two hybrid zones analyzed with newhybrids.

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