RAD in the realm of next-generation sequencing technologies

Authors


H. C. Rowe, Fax: (604) 822 6089;
E-mail: roweheat@gmail.com

Abstract

The first North American RAD Sequencing and Genomics Symposium, sponsored by Floragenex (http://www.floragenex.com/radmeeting/), took place in Portland, Oregon (USA) on 19 April 2011. This symposium was convened to promote and discuss the use of restriction-site-associated DNA (RAD) sequencing technologies. RAD sequencing is one of several strategies recently developed to increase the power of data generated via short-read sequencing technologies by reducing their complexity (Baird et al. 2008; Huang et al. 2009; Andolfatto et al. 2011; Elshire et al. 2011). RAD sequencing, as a form of genotyping by sequencing, has been effectively applied in genetic mapping and quantitative trait loci (QTL) analyses in a range of organisms including nonmodel, genetically highly heterogeneous organisms (Table 1; Baird et al. 2008; Baxter et al. 2011; Chutimanitsakun et al. 2011; Pfender et al. 2011). RAD sequencing has recently found applications in phylogeography (Emerson et al. 2010) and population genomics (Hohenlohe et al. 2010). Considering the diversity of talks presented during this meeting, more developments are to be expected in the very near future.

Table 1.   Published studies using RAD sequencing
Organism by applicationReferences
Genetic mapping
 Three-spined stickleback (Gasterosteus aculeatus)Baird et al. (2008)
 Diamondback moth (Plutella xylostella)Baxter et al. (2011)
 Barley (Hordeum vulgare)Chutimanitsakun et al. (2011)
 Perennial Ryegrass (Lolium perenne)Pfender et al. (2011)
 Neurospora crassaBaird et al. (2008)
Population genomics
 Three-spined stickleback (Gasterosteus aculeatus)Hohenlohe et al. (2010)
Phylogeography
 Pitcher plant mosquito (Wyeomyia smithii)Emerson et al. (2010)
Whole-genome sequencing
 Escherichia coliEtter et al. (2011)

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