Present address: Institut für Biologie – Botanik, Freie Universität Berlin, Altensteinstraße 6, 14195, Berlin, Germany.
Genome-wide association analysis of clinical vs. nonclinical origin provides insights into Saccharomyces cerevisiae pathogenesis
Article first published online: 31 AUG 2011
© 2011 Blackwell Publishing Ltd
Volume 20, Issue 19, pages 4085–4097, October 2011
How to Cite
MULLER, L. A. H., LUCAS, J. E., GEORGIANNA, D. R. and McCUSKER, J. H. (2011), Genome-wide association analysis of clinical vs. nonclinical origin provides insights into Saccharomyces cerevisiae pathogenesis. Molecular Ecology, 20: 4085–4097. doi: 10.1111/j.1365-294X.2011.05225.x
- Issue published online: 26 SEP 2011
- Article first published online: 31 AUG 2011
- Received 22 April 2011; revision received 8 June 2011; accepted 17 June 2011
- clinical origin;
- environmental association;
- genome-wide association analysis;
- Saccharomyces cerevisiae;
- tiling array
Because domesticated Saccharomyces cerevisiae strains have been used to produce fermented food and beverages for centuries without apparent health implications, S. cerevisiae has always been considered a Generally Recognized As Safe (GRAS) microorganism. However, the number of reported mucosal and systemic S. cerevisiae infections in the human population has increased and fatal infections have occurred even in relatively healthy individuals. In order to gain insight into the pathogenesis of S. cerevisiae and improve our understanding of the emergence of fungal pathogens, we performed a population-based genome-wide environmental association analysis of clinical vs. nonclinical origin in S. cerevisiae. Using tiling array-based, high-density genotypes of 44 clinical and 44 nonclinical S. cerevisiae strains from diverse geographical origins and source substrates, we identified several genetic loci associated with clinical background in S. cerevisiae. Associated polymorphisms within the coding sequences of VRP1, KIC1, SBE22 and PDR5, and the 5′ upstream region of YGR146C indicate the importance of pseudohyphal formation, robust cell wall maintenance and cellular detoxification for S. cerevisiae pathogenesis, and constitute good candidates for follow-up verification of virulence and virulence-related factors underlying the pathogenicity of S. cerevisiae.