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Appendix S1 Success rate of species identification and corresponding 95% confidence intervals for total queries from both singletons and non-singletons, from singletons, and from non-singletons with leave-one-out simulations (detailed).

Appendix S2 (a–d) Detailed species assignments for Neotropical bats (Clare et  al. 2006) based on COI sequences for 500 replications of queries [100 for Bayesian and NJ + Bootstrap (BootstrapNJ) methods] with five different algorithms [Bayesian algorithm (Munch et  al. 2008a), BootstapNJ algorithm (Munch et  al. 2008a), BCM algorithm (‘best close match’, Meier et  al. 2006), MD (minimum distance), MD + fuzzy membership (developed in this study)].

Appendix S3 (a–d) Detailed species assignments for Pacific Canadian marine fish (Steinke et  al. 2009) based on COI sequences for 500 replications of queries (100 for Bayesian and BootstapNJ methods) with five different algorithms [Bayesian algorithm (Munch et  al. 2008a), BootstapNJ algorithm (Munch et  al. 2008a), BCM algorithm (‘best close match’, Meier et  al. 2006), MD (minimum distance), MD + fuzzy membership (developed in this study)].

Appendix S4 (a–d) Detailed species assignments for temperate Europe butterfly (Dinca et  al. 2011) based on COI sequences for 500 replications of queries (100 for Bayesian and BootstapNJ methods) with five different algorithms [Bayesian algorithm (Munch et  al. 2008a), BootstapNJ algorithm (Munch et  al. 2008a), BCM algorithm (‘best close match’, Meier et  al. 2006), MD (minimum distance), MD + fuzzy membership (developed in this study)].

Appendix S5 (a–d) Detailed species assignments for 449 species of Diptera (Meier et  al. 2006) based on COI sequences for 500 replications of queries (100 for Bayesian and BootstapNJ methods) with five different algorithms [Bayesian algorithm (Munch et  al. 2008a), BootstapNJ algorithm (Munch et  al. 2008a), BCM algorithm (‘best close match’, Meier et  al. 2006), MD (minimum distance), MD  +  fuzzy membership (developed in this study)].

FilenameFormatSizeDescription
MEC_5235_sm_AppendixS1.xls25KSupporting info item
MEC_5235_sm_AppendixS2.dat198KSupporting info item
MEC_5235_sm_AppendixS3.dat229KSupporting info item
MEC_5235_sm_AppendixS4.dat272KSupporting info item
MEC_5235_sm_AppendixS5.dat309KSupporting info item

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