Table S1 Location of sampled isolates and mating types.

Table S2 Locus by locus heterozygosity and details within supercontigs.

Table S3 Representative calculation of area and effects of isolates grown at 31 degrees.

Table S4 Trait variation between P. chrysogenum and the Fleming species.

Fig. S1 Isolates from the Alexander Fleming Laboratory Museum.

Fig. S2 Placement of Scott et al. (2004) clades based on benA phylogeny.

Fig. S3 Spatial principal component analysis (sPCA) plots for P. chrysogenum.

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