Fig. S1 Graphical representation of five scenarios m0odelled in DIY ABC for demographic expansion patterns of four genetic clusters with the posterior probabilities (95% interval are in brackets). Parameters estimated are effective population size (N), divergence time (t), rate of admixture (r). In scenarios 1, 2 and 3, genetic cluster ‘North’ is the result of admixture between two populations at a rate ra. In scenarios 4 and 5, genetic cluster ‘North’ diverged from the demographic expansion of populations in southern BC and the Rocky Mountains.

Fig. S2 Assignment of 293 isolates from 19 populations into each of the optimal clusters identified using structure. Two clusters (= 2) were identified from 19 populations based on calculations from Evanno et al. (2006), where members of the H and FSJ populations formed a unique cluster. A second structure analysis on the remaining 17 populations revealed three additional groups (= 3) resembling the ‘North’, ‘SBC’ and ‘Rocky’ clusters in tess, but showing high levels of admixtures.

Fig. S3 Assignment of 293 isolates from 19 populations to each of the five optimal clusters identified using baps, in parallel to the four clusters recognized in tess.

Fig. S4 Principal Coordinates Analysis (PCoA) among 19 populations based on Nei’s unbiased genetic distance using GenAlEx. The first and third axes accounted for 45% and 12% of the variance respectively.

Fig. S5 Plot of isolation by distance for the entire population (blue diamonds), the genetic cluster ‘North’ (green triangles) and the genetic cluster ‘Rocky’ (red squares).

Table S1 Prior distributions and the demographic and historical parameters estimated in DIY ABC analyses. (N, effective population size; t, divergence time in terms of generation time; r, rate of admixture; μ, mutation rate; P, parameter of geometric distribution; μSNI, mutation rate in single nucleotide instability).

Table S2 Microsatellite profiles for 335 single-spore isolates (isolates in grey were not included in the clone-corrected data set).

Table S3 Admixture analysis for 293 single-spore isolates of G.  clavigera from 19 locations: average membership coefficients in four clusters inferred with tess.

Table S4 amova results for the 12 G. clavigera populations obtained in 2007–2008.

Table S5 Pairwise FST calculated with Arlequin (assessed after 1000 permutations). *P < 0.05; **P < 0.01.

Appendix S1 Output file from samova for K = 4.

MEC_5366_sm_SupportingInformation.pdf979KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.